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Created with Fabric.js 1.4.5 Goat Industry is an important livelihood for rural farmers in Southeast Asia, particularly in the Philippines. However, gastrointestinal parasitism mainly caused by Haemonchus contortus remains to be a major constraint to its efficient production. This parasite was studied to have grown resistance to a wide variety of chemical anthelmintics over continued course of treatment. This problem spurred the search for alternatives, one of which is a dewormer derived from plants. Evaluation of the Anthelmintic Properties of Perr. Crude Extract Against Eggs And Larvae Evaluation of the Anthelmintic Properties of Perr. Crude Extract Against Eggs And Larvae Haemonchus contortus Haemonchus contortus In vitro In vitro Ceriops Ceriops tagal tagal Erlinda A. Vasquez, Tomas J. Fernandez, Jr., Shiela N. Romero, Joji Marie Y. Teves | PhilRootCrops Research Center | Department of Veterinary Medicine | Visayas State University, Baybay City, Leyte | Quick Facts: Approximately 70 % of the world's poor live in rural areas, where agriculture is the main source of income and employment [1]. In the Philippines, about 45%of total population are employed as rural farmers. That's about 2 out of 5 Filipinos [2]. It is estimated that 17% of those farmers are dependent on livestock as income source with the Goat Industry contributing about 3.4% [3]. In figures, the Philippines have a total of 143 small scale backyard farms with an average of 8.7 does per farm and about 34 commercial farms having 33 does per farm on average [4]. However, about $ 3 M of estimatedannual loss due to parasite- relatedcomplications (e.g. poor milk and meat quality, mortality) was reported.Haemonchus contortus was the topcontributing parasite [3]. Methodology: Results: Conclusions and Recommendations: References:[1][2][3] Montes N.D., Zapata Jr N.R., Alo A.M.P. and Mullen J.D. 2008. Management of internal parasites in goats in the Philippines. ACIAR Impact Assessment Series Report No. 57, 44pp[4] NGFPP Terminal Report, 2012:[5][6] Araujo, R. 2002. A literature review of the effects of natural and mechanical alterations on mangrove. Division of Marine Affairs and Policy, Rosenstiel School of Marine and Atmospheric Science. University of Miami, Florida. 9 p.[7] Juhnke, J., J. Miller, J.O. Hall, F.D. Provenza and J.J. Villalba. 2012. Preference for condensed tannins by sheep in response to challenge infection with Haemonchus contortus.Vet. Par. 188(1-2):104-114 STEP 1 STEP 2 STEP 3 Extract Preparation Egg-hatch Assay Larval motility Assay 1.a. Oven-dry bark (40deg C) 1.b. Pulverize dried bark (Wiley Lab Mill) 1.c. Infuse in Ethanol ( 48 hours) 1.d. Filter 1.e. Concentrate at 300 rpm 60 deg C at Rotavapor = Ceriops tagal Crude extract 2.a Source out eggs from adult H.c. 2.b. Egg-hatch assay set-up 2.c. Treatment 2.d. Monitoring 2.e. Calculations 3.a. Culture larvae from artificially infected goats 3.b. LMA set-up 3.c. Treatment 3.d. Hourly monitoring 3.e. Calculations The results suggested that the potential value of CtCE as good alternative plant- based dewormer beconsidered for in vivo trials to further verify the plant extract's efficacy. 2 out 5 Ceriops tagal, a mangrove, was preferred as source of raw material in the study because of its significant tannin content.Occasional pruning as practiced by mangrove farmers leave pruned barks into recycled use for different purposes. The bioactive compounds in the extract with tannin- as active compound, works by diffusion through eggshells and trans-cuticular absorption.Then, the compounds bind to the free protein available for the first-stage larvae for nutrition and thus, depriving the larvae of food, then provoking their death in embryonated eggs. Upon treatment of CtCE on the larvae, the extract triggered its natural defense mechanism makingit insusceptible to the possible effects of the extract. CtCE has better activity on the eggs compared to the third stage larvae since CtCE can easily diffuse through the developing protein eggshells than the complex protein cuticles of larvae, through a tannin-protein interaction. The ovicidal and morbidity indu-cing activity of CtCE was attributed to the condensed tannins mainly as an active compound of C.tagal bark and trace amounts of other bioactive compounds in the extract. [3] CtCE
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