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Created with Fabric.js 1.4.5 Michelle Ann J. Dela Cuesta, Princess C. Lapid, Nordalyn B. Pedroche, Ph.D. Results Methodology Introduction Department of Biology, School of Science and Engineering, Ateneo de Manila University, Quezon City 1108 Nematicidal Effects of Origanum vulgare Leaf Extracts Against the Root-Knot Nematode Meloidogyne spp. The root-knot nematode, Meloidogyne incognita is a major plant parasite that reduces the quality and quantity of annual and perennial plants. Use of chemicals to control parasitic nematodes is expensive and is economically practical only for crops with high value. In addition, chemical control maypromote potential hazard to human health and environment.The inconveniences lead to identification of natural productscapable of being nematicidal agents. There is a need for aremedy that is both affordable and abundant for farmers. This study was carried out to evaluate oregano leaf extract for its nematicidal property against root-knot nematode Meloidogyne spp. Egg mass extraction Plant extract preparation Laboratory exposureof egg mass Analysis of data Fungi isolation and identification Acknowledgment Conclusion Origanum vulgare extract used in the experiment did not contribute to larval mortality because hatching still occurred on the tested treatment concentration. However, it was shown that the longer exposure of nematodes to the extract decreases hatching. Despite the extract being unable to prevent hatching of egg masses, it was able to affect the motility of the nematodes. The data presented has led to this conclusion since most nematodes present were inactive. It was also concluded that fungal growth contributed to the rate of nematode hatching by paralyzing the nematode eggs. This research would not be possible without our thesis adviser, Dr. NordalynPedroche, and our defense panelists, Ms. Rhea Abisado and Mr. Jan Lorie Robil.Our many thanks are also extended to the laboratory technicians of the DepartmentBiology and to our friends who worked with us in the laboratory. FIG. 1. Nematicidal activity oforegano extracts during the first trial Day 1 (A), Day 2 (B),second trial Day 1 (C), and Day 2 (D). It was seen that the treatment showed response in terms of motility, as shown by the high number of inactive nematodes. However,the result in (C) and (D) did not align with (A) and (B) and fungal growth was observed. FIG. 2. Morphographical characteristics of fungi isolated from the eggs of root-knot nematode, Meloidogyne spp. Conidiophore formation of Verticillium spp. (A & B), Helicocephalum spp. (C &D), Amblyosporium spp. (E, F, G, & H), Hansfordia spp. (I & J),and Penicilliumspp. (K, L, & M). References Barnett HL and Hunter BB. Illustrated genera of imperfect fungi, fourth edition.Amer Phytopathological Society. 4: 1-240.Oka Y, Nacar S, Putievsky E, Ravid U, Yaniv Z, Spiegel Y. 2000. Nematicidal activity of essential oils and their components against the root-knot nematode. US National Library of Medicine National Institutes of Health. 90 (7): 710-715.Perry R, Moens M, Starr J. 2009. Root-knot nematodes. MPG Books Group. United Kingdom.
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